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SIGMA-ALDRICH

Thiazolyl Blue Tetrazolium Bromide

  • Item # 46AA90
  • My Part #
  • Mfr. Model # M2128-1G
  • Catalog Page # N/A
  • UNSPSC # 12191602
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  • Shipping Weight 3.0 lbs.
Country of Origin USA Country of Origin is subject to change.
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Product Details

Thiazolyl Blue Tetrazolium Bromide may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

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Technical Specs

  • Item Thiazolyl Blue Tetrazolium Bromide
  • Concentration 98%
  • CAS Number 298-93-1
  • Molecular Weight 414.32
  • Chemical Formula C18H16BrN5S
  • Chemical Container Material Clear Glass
  • Chemical Container Type Vial
  • Chemical Container Size 1g
  • Chemical Family Type Solvents
  • Shelf Life 24 mo.
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Compliance and Restrictions

  • Hazardous material - Non-Flammable Gas


  • This item is restricted for international sale.

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